Print   |   A-  A  A+

Department Immunology and Cell Biology

Cellular Allergology


  • Isolation of human cell populations from peripheral blood:
    • basophilic granulocytes (purity 98 - 100%),
    • monocytes
    • lymphocytes (CD4+ T cells, naïve T cells)
  • Cultivation of different cell lines: KU812, HEK, RBL, J558-CD40L, High-Five
  • Quantitative detection of cytokines, IgE, IgG, histamine, hexosaminidase and leukotrienes in culture supernatants by ELISA (and Cytometric Bead Array (BD) for cytokine detection)
  • FACS analysis of surface antigens and intracellular staining of cytokines using LSRII
  • In vitro culture of parasitic worms, especially Schistosoma mansoni
  • Conditioning of human monocyte-derived dendritic cells and polarization of naïve CD4+ T cells co-cultured with differentially conditioned DC
  • Fc receptor co-aggregation (FcRI / FcγRIIB)
  • Dot immunobinding assay for quantitative detection of IgG subclasses IgG1-4
  • Qualitative and quantitative electrophoretic separation techniques:
    • isoelectrofocussing,
    • Rotophor electrophoresis,
    • SDS-PAGE
  • Double immunodiffusion assay (Ouchterlony)
  • Immuno- and affinoblotting techniques
  • Protein purification via affinity, ion exchange, and size exclusion chromatography
  • Methods for detection of circulating immune complexes
  • Radioactive labeling of proteins / autoradiography
  • Purification of immunoglobulins from serum or culture supernatants (e.g. monoclonal antibodies)
  • Isolation of mRNA from cells and tissues ( for example S. mansoni eggs)
  • RT-PCR
  • PCR, cloning techniques, in vitro mutagenesis, DNA-sequencing
  • Construction and screening of cDNA  expression libraries (immunoscreening, hybridisation)
  • Expression of recombinant proteins in prokaryotic (E. coli) and eukaryotic (HEK cells, insect cells (High-Five)) systems.
  • Purification of  recombinant proteins via affinity chromatography (Ni-NTA, Amylose, specific antibodies)
  • Refolding of denatured recombinant proteins