Programmbereich Infektionen
Zelluläre Mikrobiologie
Techniques
Infection biology
- Studies under BSL3 safety conditions
- Infection models for Tuberculosis, Salmonellosis, Streptococcus pyogenes, S. pneumoniae
- Natural infection pathways (by aerosol, orally; BSL3)
- Vaccination and drug treatment protocols
- Colony forming units
- Lung microbiota analysis, FISH
- In vivo imaging (BSL3)
- In vitro infection models of mouse and human primary cells
Infection Immunology
- FACS analysis and sorting (BSL2 and 3)
- Histology, immuno histology
- Cytokine / chemokine assays
- T cell assays
- Real-time PCR
- Small lipid mediator analysis
Cellular microbiology
- Intracellular trafficking studies, confocal microscopy
- Life cell imaging (BSL2 and 3)
- Primary cell cultures (human, mouse; macrophages, neutrophils, dendritic cells, lymphocytes, lung epithelial cells)
- Subcellular fractionation, phagosome isolation and characterization
- Cell death assays
- Transfection, siRNA based knock down
Biochemistry
- SDS-PAGE, western blot, pull down
- Liposomes, liposome leakage assays
- Glycolipid isolation, labeling
- Thin layer chromatography