Programmbereich Infektionen

Zelluläre Mikrobiologie

Mission    Projekte    Drittmittel    Methoden    Publikationen    Mitarbeiter/innen

Techniques


Infection biology

  • Studies under BSL3 safety conditions
  • Infection models for Tuberculosis, Salmonellosis, Streptococcus pyogenes, S. pneumoniae
  • Natural infection pathways (by aerosol, orally; BSL3)
  • Vaccination and drug treatment protocols
  • Colony forming units
  • Lung microbiota analysis, FISH
  • In vivo imaging (BSL3)
  • In vitro infection models of mouse and human primary cells

    
Infection Immunology

  • FACS analysis and sorting (BSL2 and 3)
  • Histology, immuno histology
  • Cytokine / chemokine assays
  • T cell assays
  • Real-time PCR
  • Small lipid mediator analysis

    
Cellular microbiology

  • Intracellular trafficking studies, confocal microscopy
  • Life cell imaging (BSL2 and 3)
  • Primary cell cultures (human, mouse; macrophages, neutrophils, dendritic cells, lymphocytes, lung epithelial cells)
  • Subcellular fractionation, phagosome isolation and characterization
  • Cell death assays
  • Transfection, siRNA based knock down

    
Biochemistry

  • SDS-PAGE, western blot, pull down
  • Liposomes, liposome leakage assays
  • Glycolipid isolation, labeling
  • Thin layer chromatography