Priority Research Area Infections

Infection Immunology

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  • Biochemical methods
  • Cultivation of hybridoma, production and purification of monoclonal antibodies
  • Cultivation, preparation and identification of BSL2 and BSL3 pathogens (e.g. Mycobacterium tuberculosis or Trypanosoma cruzi)
  • Infection of experimental mice (subcutaneous, intravenous, intraperitoneal, aerosol infection with mycobacteria)
  • In vitro differentiation, infection and analysis of bone marrow derived macrophages and dendritic cells
  • In vitro differentiation of TH1, TH2 and TH17 cells
  • Isolation of organs from experimental mice, preparation of single cell suspension and cell sorting
  • Isolation of cells and granulomas from perfused lungs and livers of infected animals
  • Magnetic cell sorting
  • Flowcytometry and immunofluorescence
  • Histological and immunohistochemical techniques
  • Confocal microscopy
  • Quantitative RT-PCR, real time PCR
  • Detection of antibodies, cytokines and chemokines intermediates in tissue culture supernatants, tissue homogenates and serum/plasma
  • Cytometric bead arrays
  • Determination of reactive nitrogen intermediates and quantification of arginase activity
  • Cytokine bioassays
  • Functional T cell assays (proliferation, antigen-specific recall and cytokine-secretion assays, ELISPOT, CTL assays, preparation of Mtb-specific class I and class II tetramers and staining of specific CD4+ and CD8+ T cells)