Priority Research Area Infections

Molecular and Experimental Mycobacteriology

Mission   Projects   Funding   Techniques   Publications    Staff

Techniques

  • Standardized culture systems for investigation of clinical M. tuberculosis complex isolates.
  • Standardized methods for isolation of genomic DNA, RNA and intracellular protein.
  • Molecular tools for analyses of genomic DNA and RNA (Agarose Gel Electrophoresis, Southern Blot, PCR, Real Time PCR, DNA-Sequencing, Microarray)
  • Genotyping of M. tuberculosis complex isolates by:
  • IS6110-DNA-Fingerprint
  • Spoligotyping
  • Mycobacterial Interspersed Repetitive Units Variable Number Repeats (MIRU-VNTR)-typing
  • Analysis of Large Sequence Polymorphisms (Regions of Difference)
  • Analysis of Single Nucleotide Polymorphisms (SNPs)
  • Gene expression analysis of M. tuberculosis complex isolates by:
  • Real-Time PCR
  • Whole genome expression profiling
  • Generation of M. tuberculosis mutants (S3)
  • Bioinformatic analysis and storage of typing data by using the  Bionumerics software
  • Bioinformatic analysis of DNA sequence data  by using different software packages (SeqScape, Lasergene, GenDB)