Priority Research Area Chronic Lung Diseases

University Liaison Group Epigenetics of Chronic Lung Diseases

Mission    Projects    Funding    Techniques    Publications    Staff


Molecular biology methods

  • SDS-PAGE, Western blotting
  • PCR, preparative agarose gels,  qPCR
  • Chromatin immunoprecipitation (ChIP)
  • Dot Blot
  • Immunohistology


Genetic engineering methods (S1 and S2) and next-generation sequencing (NGS)

  • E. coli protein expression
  • Cloning vector design
  • Lipofection
  • siRNA
  • dCas driven methyltransferases (under development)
  • Pyrosequencing, RNA sequencing (pre-analytics)
  • Spatial Methylomics


Data science

  • Design of Experiments (DoE) layouts
  • RNA-Seq analyses (differential expression, enrichments)
  • DNA methylation analysis (differential methylations, eQTL)
  • Cohort design and cohort analysis (cross-sectional, longitudinal)


Cell biology methods

  • Primary cells & mammalian cell lines(?) culture(nasal epithelial cells, peripheral blood cells, wide range of lung cells)
  • Air-Liquid Interface cultures
  • Infections with human pathogens (e.g. rhinoviruses) or gene carriers (lentiviruses)
  • 3D cell migration assay (SiMA)