Priority Research Area Asthma and Allergy

Innate Immunity

Mission   Projects   Funding   Techniques   Publications  Staff



Herster, F, Bittner, Z, Archer, NK, Dickhöfer, S, Eisel, D, Eigenbrod, T, Knorpp, T, Schneiderhan-Marra, N, Löffler, MW, Kalbacher, H, Vierbuchen, T, Heine, H, Miller, LS, Hartl, D, Freund, L, Schäkel, K, Heister, M, Ghoreschi, K & Weber, ANR 2020, 'Neutrophil extracellular trap-associated RNA and LL37 enable self-amplifying inflammation in psoriasis', Nature communications, Jg. 11, Nr. 1, S. 1-13.

Komaniecka, I, Susniak, K, Chroma, A, Heine, H & Holst, O 2020, 'The Lipid A from the Lipopolysaccharide of the Phototrophic Bacterium Rhodomicrobium vannielii ATCC 17100 Revisited', INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , Jg. 22, Nr. 1.

Wang, Z, Minarsch, E-ML, Kublik, S, Heine, H, Schloter, M & Foesel, BU 2020, 'Complete Genome Sequence of Lactococcus lactis subsp. lactis G121, an Isolate with Allergy-Protective Features Derived from a Farming Environment', Microbiology resource announcements, Jg. 9, Nr. 41.

Zamyatina, A & Heine, H 2020, 'Lipopolysaccharide Recognition in the Crossroads of TLR4 and Caspase-4/11 Mediated Inflammatory Pathways', FRONTIERS IN IMMUNOLOGY, Jg. 11, S. 585146.



Bossen J, Uliczka K, Steen L, Pfefferkorn R, Mai MM, Burkhardt L, Spohn M, Bruchhaus I, Fink C, Heine H, Roeder T: An EGFR-Induced Drosophila Lung Tumor Model Identifies Alternative Combination Treatments. Mol Cancer Ther 2019, 18(9):1659-1668.

Freund I, Buhl DK, Boutin S, Kotter A, Pichot F, Marchand V, Vierbuchen T, Heine H, Motorin Y, Helm M, Dalpke AH, Eigenbrod T: 2'-O-methylation within prokaryotic and eukaryotic tRNA inhibits innate immune activation by endosomal Toll-like receptors but does not affect recognition of whole organisms. Rna 2019, 25(7):869-880.

Jappe U, Schwager C, Schromm AB, Gonzalez Roldan N, Stein K, Heine H, Duda KA: Lipophilic Allergens, Different Modes of Allergen-Lipid Interaction and Their Impact on Asthma and Allergy. Front Immunol 2019, 10:122.

Vierbuchen T, Stein K, Heine H: RNA is taking its Toll: Impact of RNA-specific Toll-like receptors on health and disease. Allergy 2019, 74(2):223-235.



Adanitsch, F, Shi, J, Shao, F, Beyaert, R, Heine, H & Zamyatina, A 2018, 'Synthetic glycan-based TLR4 agonists targeting caspase-4/11 for the development of adjuvants and immunotherapeutics' Journal of Physical Chemistry B, Bd 9, Nr. 16, S. 3957-3963. DOI: 10.1039/c7sc05323a

Borio A, Holgado A, Garate JA, Beyaert R, Heine H, Zamyatina A, ‘Disaccharide-Based Anionic Amphiphiles as Potent Inhibitors of Lipopolysaccharide-Induced Inflammation’. ChemMedChem 2018, 13(21):2317-2331.

Vierbuchen, T., Stein, K., Hein, H., ‘RNA is taking its Toll - Impact of RNA-specific Toll-like receptors on health and disease’. Allergy 2018 Nov 26. doi: 10.1111/all.13680. [Epub ahead of print].



Gorczynski, RM, Alexander, C, Brandenburg, K, Chen, Z, Heini, A, Heumann, D, Mach, J-P, Rietschel, ET, Ulmer, AJ, Zähringer, U, Yu, K & Khatri, I 2017, 'An altered REDOX environment, assisted by over-expression of fetal hemoglobins, protects from inflammatory colitis and reduces inflammatory cytokine expression' INTERNATIONAL IMMUNOPHARMACOLOGY , Bd 50, Nr. (2017), S. 69-76.

Lindner, K, Ströbele, M, Schlick, S, Webering, S, Jenckel, A, Kopf, J, Danov, O, Sewald, K, Buj, C, Creutzenberg, O, Tillmann, T, Pohlmann, G, Ernst, H, Ziemann, C, Hüttmann, G, Heine, H, Bockhorn, H, Hansen, T, König, P & Fehrenbach, H 2017, 'Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons' Particle and Fibre Toxicology, Bd 14, Nr. 1, S. 8. DOI: 10.1186/s12989-017-0189-1

Stein, K, Brand, S, Jenckel, A, Sigmund, A, Chen, ZJ, Kirschning, CJ, Kauth, M & Heine, H 2017, 'Endosomal recognition of Lactococcus lactis G121 and its RNA by dendritic cells is key to its allergy-protective effects' The Journal of allergy and clinical immunology, Bd 139, Nr. 2, S. 667-678. DOI: 10.1016/j.jaci.2016.06.018

Bang, C, Vierbuchen, T, Gutsmann, T, Heine, H & Schmitz, RA 2017, 'Immunogenic properties of the human gut-associated archaeon Methanomassiliicoccus luminyensis and its susceptibility to antimicrobial peptides' PLOS ONE, Bd 12, Nr. 10, S. e0185919. DOI: 10.1371/journal.pone.0185919

Jatzlauk, G, Bartel, S, Heine, H, Schloter, M & Krauss-Etschmann, S 2017, 'Influences of environmental bacteria and their metabolites on allergies, asthma and host microbiota' ALLERGY, Bd 72, Nr. 12, S. 1859-1867. DOI: 10.1111/all.13220

Pooe, OJ, Köllisch, G, Heine, H & Shonhai, A 2017, 'Plasmodium falciparum heat shock protein 70 lacks immune modulatory activity' PROTEIN AND PEPTIDE LETTERS, Bd 24, Nr. 6, S. 503-510. DOI: 10.2174/0929866524666170214141909



Zissler, UM, Chaker, AM, Effner, R, Ulrich, M, Guerth, F, Piontek, G, Dietz, K, Regn, M, Knapp, B, Theis, FJ, Heine, H, Suttner, K & Schmidt-Weber, CB 2016, 'Interleukin-4 and interferon-γ orchestrate an epithelial polarization in the airways' Mucosal immunology, Bd 9, Nr. 4, S. 917-26. DOI: 10.1038/mi.2015.110

Kauth, M & Heine, H 2016, 'Allergy protection by cowshed bacteria - recent findings and future prospects' Pediatric Allergy and Immunology, Bd 27, Nr. 4, S. 340-7. DOI: 10.1111/pai.12559

Ulmer, AJ, Kaconis, Y, Heinbockel, L, Correa, W, Alexander, C, Rietschel, ET, Mach, J-P, Gorczynski, RM, Heini, A, Rössle, M, Richter, W, Gutsmann, T & Brandenburg, K 2016, 'Enhancing actions of peptides derived from the γ-chain of fetal human hemoglobin on the immunostimulant activities of monophosphoryl lipid A' INNATE IMMUNITY, Bd 22, Nr. 3, S. 168-80. DOI: 10.1177/1753425916632304

Polansky, JK, Bahri, R, Divivier, M, Duitman, EH, Vock, C, Goyeneche-Patino, DA, Orinska, Z & Bulfone-Paus, S 2016, 'High dose CD11c-driven IL15 is sufficient to drive NK cell maturation and anti-tumor activity in a trans-presentation independent manner' Scientific Reports, Bd 6, S. 19699. DOI: 10.1038/srep19699



Martinez de Tejada G, Heinbockel L, Ferrer-Espada R, Heine H, Alexander C, Bárcena-Varela S, Goldmann T, Correa W, Wiesmüller KH, Gisch N, Sánchez-Gómez S, Fukuoka S, Schürholz T, Gutsmann T, Brandenburg K. Lipoproteins/peptides are sepsis-inducing toxins from bacteria that can be neutralized by synthetic anti-endotoxin peptides. Sci Rep. 2015 Sep 22;5:14292. doi: 10.1038/srep14292.

Garate, JA, Stöckl, J, Del Carmen Fernández-Alonso, M, Artner, D, Haegman, M, Oostenbrink, C, Jiménez-Barbero, J, Beyaert, R, Heine, H, Kosma, P & Zamyatina, A 2015, 'Anti-endotoxic activity and structural basis for human MD-2•TLR4 antagonism of tetraacylated lipid A mimetics based on βGlcN(1↔1)αGlcN scaffold' INNATE IMMUNITY, Bd 21, Nr. 5, S. 490-503.

Heinbockel, L, Palacios-Chaves, L, Alexander, C, Rietschel, E, Behrends, J, Correa, W, Fukuoka, S, Gutsmann, T, Ulmer, AJ & Brandenburg, K 2015, 'Mechanism of Hbγ-35-induced an increase in the activation of the human immune system by endotoxins' INNATE IMMUNITY, Bd 21, Nr. 3, S. 305-13.

Kallsen, K, Zehethofer, N, Abdelsadik, A, Lindner, B, Kabesch, M, Heine, H & Roeder, T 2015, 'ORMDL deregulation increases stress responses and modulates repair pathways in Drosophila airways' The Journal of allergy and clinical immunology., 10.1016/j.jaci.2015.04.009



Bang, C, Weidenbach, K, Gutsmann, T, Heine, H & Schmitz, RA 2014, 'The Intestinal Archaea Methanosphaera stadtmanae and Methanobrevibacter smithii Activate Human Dendritic Cells' PLOS ONE, Bd 9, Nr. 6, S. e99411.

Claes, A-K, Steck, N, Schultz, D, Zähringer, U, Lipinski, S, Rosenstiel, P, Geddes, K, Philpott, DJ, Heine, H & Grassl, GA 2014, 'Salmonella enterica serovar Typhimurium ΔmsbB Triggers Exacerbated Inflammation in Nod2 Deficient Mice' PLOS ONE, Bd 9, Nr. 11, S. e113645.

Hogendorf, WFJ, Gisch, N, Schwudke, D, Heine, H, Bols, M & Pedersen, CM 2014, 'Total Synthesis of Five Lipoteichoic acids of Clostridium difficile' CHEMISTRY - A EUROPEAN JOURNAL, Bd 20, Nr. 42, S. 13511-13516.

Mansour, NM, Heine, H, Abdou, SM, Shenana, ME, Zakaria, MK & Êl-Diwany, A 2014, 'Isolation of Enterococcus faecium NM113, Enterococcus faecium NM213 and Lactobacillus casei NM512 as novel probiotics with immunomodulatory properties' Microbiology and Immunology, Bd 58, S. 559-569.



Harb H, van Tol EA, Heine H, Braaksma M, Gross G, Overkamp K, Hennen M, Alrifai M, Conrad ML, Renz H, Garn H. Neonatal supplementation of processed supernatant from Lactobacillus rhamnosus GG improves allergic airway inflammation in mice later in life. Clin Exp Allergy. 2013 Mar;43(3):353-64.

Hagner S, Harb H, Zhao M, Stein K, Holst O, Ege MJ, Mayer M, Matthes J, Bauer J, von Mutius E, Renz H, Heine H, Pfefferle PI, Garn H. Farm-derived Gram-positive bacterium Staphylococcus sciuri W620 prevents asthma phenotype in HDM- and OVA-exposed mice. Allergy. 2013 Mar;68(3):322-9.

Roeder, T, Isermann, K, Kallsen, K, Uliczka, K & Wagner, C 2013, 'A Drosophila Asthma Model – What the Fly Tells Us About Inflammatory Diseases of the Lung.'. in Recent advances on model hosts. Bd. 710, Springer, S. 37-47.

Warmbold C, Uliczka K, Rus F, Suck R, Petersen A, Silverman N, Ulmer AJ, Heine H, Roeder T. Dermatophagoides pteronyssinus major allergen 1 activates the innate immune response of the fruit fly Drosophila melanogaster. J Immunol. 2013 Jan 1;190(1):366-71.



Kallsen K, Andresen E, Heine H. Histone Deacetylase (HDAC) 1 Controls the Expression of Beta Defensin 1 in Human Lung Epithelial Cells. PLOS ONE 2012;7(11):e50000. Open Access

Fischer K, Vinogradov E, Lindner B, Heine H, Holst O. The structure of the extracellular teichoic acids from the allergy-protective bacterium Lactococcus lactis G121. BIOLOGICAL CHEMISTRY 2012 Aug;393(8): 749-55.

Brade L, Heine H, Raina S, Klein G, di Padova F, Brade H, Müller-Loennies S. Immunization with an anti-idiotypic antibody against the broadly lipopolysaccharide-reactive antibody WN1 222-5 induces Escherichia coli R3-core-type specific antibodies in rabbits. INNATE IMMUNITY 2012 Feb;18(2):279-93.

Farhat K, Ulmer AJ, Jungi TW. A potential test system for detecting contaminations by bacterial lipoproteins. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 2012 Jan;145(1-2):66–73.

Pedersen CM, Figueroa-Perez I, Ulmer AJ, Zähringer U, Schmidt RR. Synthesis of the repeating unit of the lipoteichoic acid of Streptococcus pneumoniae. TETRAHEDRON 2012 Jan;68(4):1052–1061.



Fischer K, Stein K, Ulmer AJ, Lindner B, Heine H, Holst O. Cytokine-inducing lipoteichoic acids of the allergy-protective bacterium Lactococcus lactis G121 do not activate via Toll-like receptor 2. GLYCOBIOLOGY 2011 Dec;21(12):1588-1595.

Andresen E, Günther G, Bullwinkel J, Lange C, Heine H. Increased expression of beta-defensin 1 (DEFB1) in chronic obstructive pulmonary disease. PLoS ONE 2011 Jul;6(7):e21898. Open Access

Rossol M, Heine H, Meusch U, Quandt D, Klein C, Sweet MJ, Hauschildt S. LPS-induced cytokine production in human monocytes and macrophages. CRITICAL REVIEWS IN IMMUNOLOGY 2011;31(5):379-446.

Heine H. TLRs, NLRs and RLRs: Innate sensors and their impact on allergic diseases - A current view. IMMUNOLOGY LETTERS 2011 Sept;139(1-2):14-24 Epub 2011 Apr 30.

Gisch N, Buske B, Heine H, Lindner B, Zähringer U. Synthesis of biotinylated muramyl tripeptides with NOD2-stimulating activity. BIOORGANIC & MEDICINAL CHEMISTRY; 2011 Jun 21(11):3362-6. Epub 2011 Apr 9.

Andresen E, Lange C, Strodthoff D, Goldmann T, Fischer N, Sahly H, Branscheid D, Heine H. S100A7/psoriasin expression in the human lung: unchanged in patients with COPD, but upregulated upon positive S. aureus detection. BMC Pulmonary Medicine 2011 Feb;11:10. Open Access



Debarry J, Hanuszkiewicz A, Stein K, Holst O, Heine H. The allergy-protective properties of Acinetobacter lwoffi F78 are imparted by its lipopolysaccharide. ALLERGY 2010 Jun;65(6):690-697.

Dromann D, Rupp J, Rohmann K, Osbahr S, Ulmer AJ, Marwitz S, Roschmann K, Abdullah M, Schultz H, Vollmer E, Zabel P, Dalhoff K, Goldmann T. The TGF-beta-Pseudoreceptor BAMBI is strongly expressed in COPD lungs and regulated by nontypeable Haemophilus influenzae. RESPIRATORY RESEARCH 2010 Mai;11(1):67. Open Access

Pedersen C, Figueroa-Perez I, Lindner B, Ulmer A, Zahringer U, Schmidt R. Total Synthesis of Lipoteichoic Acid of Streptococcus pneumoniae. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2010 Mar;49(14):2585-2590.

Farhat K, Riekenberg S, Jung G, Wiesmüller K, Jungi TW, Ulmer AJ. Identification of full length bovine TLR1 and functional characterization of lipopeptide recognition by bovine TLR2/1 heterodimer. VETERINARY RESEARCH 2010 Jul;41(3):34.



Riekenberg S, Farhat K, Debarry J, Heine H, Jung G, Wiesmuller K, Ulmer A. Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide. FEBS JOURNAL 2009 Feb;276(3):649-659.



Hanuszkiewicz A, Kaczynski Z, Lindner B, Goldmann T, Vollmer E, Debarry J, Heine H, Holst O. Structural Analysis of the Capsular Polysaccharide from Acinetobacter lwoffii F78. EUROPEAN JOURNAL OF ORGANIC CHEMISTRY 2008 Dec;(36):6183-6188.

Haase H, Ober-Bloebaum J, Engelhardt G, Hebel S, Heit A, Heine H, Rink L. Zinc Signals Are Essential for Lipopolysaccharide-Induced Signal Transduction in Monocytes. JOURNAL OF IMMUNOLOGY 2008 Nov;181(9):6491-6502.

Hanuszkiewicz A, Hubner G, Vinogradov E, Lindner B, Brade L, Brade H, Debarry J, Heine H, Holst O. Structural and Immunochemical Analysis of the Lipopolysaccharide from Acinetobacter Iwoffii F78 Located Outside Chlamydiaceae with a Chlamydia-Specific Lipopolysaccharide Epitope. CHEMISTRY-A EUROPEAN JOURNAL 2008 Oct;14(33):10251-10258.

Libani I, Guy E, Melchiori L, Schiro R, Ramos P, Breda L, Scholzen T, Chadburn A, Liu Y, Kernbach M, Baron-Luhr B, Porotto M, de Sousa M, Rachmilewitz E, Hood J, Cappellini D, Giardina P, Grady R, Gerdes J, Rivella S. Decreased differentiation of erythroid cells exacerbates ineffective erythropoiesis in beta-thalassemia. BLOOD 2008 Aug;112(3):875-885.

Vogel K, Blumer N, Korthals M, Mittelstadt J, Garn H, Ege M, von Mutius E, Gatermann S, Bufe A, Goldmann T, Schwaiger K, Renz H, Brandau S, Bauer J, Heine H, Holst O. Animal shed Bacillus licheniformis spores possess allergy-protective as well as inflammatory properties. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY 2008 Aug;122(2):307-312.

Zahringer U, Lindner B, Inamura S, Heine H, Alexander C. TLR2 - promiscuous or specific? A critical re-evaluation of a receptor expressing apparent broad specificity. IMMUNOBIOLOGY 2008 Mar;213(3-4):205-224.

Farhat K, Riekenberg S, Heine H, Debarry J, Lang R, Mages J, Buwitt-Beckmann U, Roeschmann K, Jung G, Wiesmuller K, Ulmer A. Heterodimerization of TLR2 with TLR1 or TLR6 expands the ligand spectrum but does not lead to differential signaling. JOURNAL OF LEUKOCYTE BIOLOGY 2008 Mar;83(3):692-701.



von Bulow V, Dubben S, Engelhardt G, Hebel S, Plumakers B, Heine H, Rink L, Haase H. Zinc-dependent suppression of TNF-alpha production is mediated by protein kinase A-induced inhibition of Raf-1, I kappa B kinase beta, and NF-kappa B. JOURNAL OF IMMUNOLOGY 2007 Sep;179(6):4180-4186.

Rahmanzadeh R, Huttmann G, Gerdes J, Scholzen T. Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis. CELL PROLIFERATION 2007 Jun;40(3):422-430.

Heine H, Gronow S, Zamyatina A, Kosma P, Brade H. Investigation on the agonistic and antagonistic biological activities of synthetic Chlamydia lipid A and its use in in vitro enzymatic assays. JOURNAL OF ENDOTOXIN RESEARCH 2007 Apr;13(2):126-132.

Walter S, Letiembre M, Liu Y, Heine H, Penke B, Hao W, Bode B, Manietta N, Walter J, Schulz-Schaffer W, Fassbender K. Role of the toll-like receptor 4 in neuro-inflammation in Alzheimer's disease. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 2007;20(6):947-956.


2003 – 2006 (Selection)

Buwitt-Beckmann U, Heine H, Wiesmüller K, Jung G, Brock R, Akira S, Ulmer AJ. TLR1- and TLR6-independent recognition of bacterial lipopeptides. JOURNAL OF BIOLOGICAL CHEMISTRY 2006 Apr;281(14):9049-9057.

Loseke S, Grage-Griebenow E, Heine H, Wagner A, Akira S, Bauer S, Bufe A. In vitro-generated viral double-stranded RNA in contrast to polyinosinic: Polycytidylic acid induces interferon-alpha in human plasmacytoid dendritic cells. SCANDINAVIAN JOURNAL OF IMMUNOLOGY 2006 Apr;63(4):264-274.

Vermi W, Facchetti F, Riboldi E, Heine H, Scutera S, Stornello S, Ravarino D, Cappello P, Giovarelli M, Badolato R, Zucca M, Gentili F, Chilosi M, Doglioni C, Ponzi A, Sozzani S, Musso T. Role of dendritic cell-derived CXCL13 in the pathogenesis of Bartonella henselae B-rich granuloma. BLOOD 2006 Jan;107(2):454-462.

Liu Y, Walter S, Stagi M, Cherny D, Letiembre M, Schulz-Schaeffer W, Heine H, Penke B, Neumann H, Fassbender K. LPS receptor (CD14): a receptor for phagocytosis of Alzheimer's amyloid peptide. BRAIN 2005 Aug;128:1778-1789.

Manukyan M, Triantafilou K, Triantafilou M, Mackie A, Nilsen N, Espevik T, Wiesmullers K, Ulmer A, Heine H. Binding of lipopeptide to CD14 induces physical proximity of CD14, TLR2 and TLR1. EUROPEAN JOURNAL OF IMMUNOLOGY 2005 Mar;35(3):911-921.

Buwitt-Beckmann U, Heine H, Wiesmuller K, Jung G, Brock R, Akira S, Ulmer A. Toll-like receptor 6-independent signaling by diacylated lipopeptides. EUROPEAN JOURNAL OF IMMUNOLOGY 2005 Jan;35(1):282-289.

Heine H, Ulmer AJ. Recognition of bacterial products by toll-like receptors. CHEMICAL IMMUNOLOGY AND ALLERGY 2005;86:99-119.

Triantafilou M, Manukyan M, Mackie A, Morath S, Hartung T, Heine H, Triantafilou K. Lipoteichoic acid and Toll-like receptor 2 internalization and targeting to the Golgi are lipid raft-dependent. JOURNAL OF BIOLOGICAL CHEMISTRY 2004 Sep;279(39):40882-40889.

Heine H, El-Samalouti V, Notzel C, Pfeiffer A, Lentschat A, Kusumoto S, Schmitz G, Hamann L, Ulmer A. CD55/decay accelerating factor is part of the lipopolysaccharide-induced receptor complex. EUROPEAN JOURNAL OF IMMUNOLOGY 2003 Mai;33(5):1399-1408.

Heine H, Lien E. Toll-like receptors and their function in innate and adaptive immunity. INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY 2003 Mar;130(3):180-192.