Priority Research Area Chronic Lung Diseases
Pulmonary Immune Diseases
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Projects
The ULG Pulmonary Immune Disease is a cooperation group between the Research Center Borstel and the Department of Rheumatology at the University of Lübeck. We consist of a clinical arm located in Lübeck and a basic research arm located at the FZB. This structure allows us a close translational interaction between 'Bench' and 'Bedside'.
G-protein coupled receptors (GPCRs) form the largest family of transmembrane receptors and are involved in the regulation of almost all physiological and pathophysiological processes. In recent years, it has been shown that the activity of various GPCRs is regulated not only by their cognate ligand(s), but also by autoantibodies that bind to a single GPCR and act specifically on that receptor as an agonist or antagonist (functional autoantibodies). According to our central hypothesis, functional autoantibodies against specific GPCRs are involved in the pathogenesis of systemic autoimmune diseases as well as asthma and COPD. We are studying systemic Sclerosis (SSc), an autoimmune disease characterized by severe pulmonary complications such as pulmonary arterial hypertension, pulmonary fibrosis, and pneumonia, as a model disease.
Functional autoantibodies to angiotensin II receptor type 1 (AT1R) as drivers of pathogenicity in systemic sclerosis
Based on our studies in SSc patient cohorts, we identified functional autoantibodies against AT1R as biomarkers for SSc whose levels correlate with disease severity and prognosis. Based on this observation, we are establishing novel animal models for SSc in which the disease is induced by immunization with human AT1R. Furthermore, we were able to generate a humanized mouse model of SSc by adoptive transfer of lymphocytes from SSc patients into immunodeficient mice. Furthermore, we succeeded for the first time in generating functional monoclonal antibodies against AT1R. By demonstrating their pathogenic effect in the lung, we could provide direct evidence of their impact on the pathophysiology of SSc. Using our models, we identify immune cells and cytokines relevant for the disease in order to understand disease mechanisms and develop new therapeutic strategies.
De-regulated cytokines as triggers of autoimmune-mediated disease manifestations in the lung
Systemic sclerosis presents in highly individualized clinical phenotypes in patients with vasculopathy-mediated pulmonary arterial hypertension (PAH) and pulmonary fibrosis as major causes of SSc-related deaths. This suggests that, in addition to autoimmunity and functional autoantibodies, other mechanisms exist that determine the individual pathology of the disease. This view is supported by our recently developed mouse model, in which autoimmunity to AT1R predominantly causes lung and skin inflammation but neither PAH nor pulmonary fibrosis is observed. In our conception, SSc and probably other chronic lung diseases develop in two sequential steps ("two-hit" hypothesis) As "first hit", autoantibodies mediate chronic local inflammation, which decides at which site the later manifestations occur. As a "second hit", specific de-regulated cytokines (provides by de-regulated cytokine producers; De-reg CP) are required to establish a specific symptom at the site of inflammation. This idea is strengthened by results from our ongoing study in IL-13-overexpressing mice, in which AT1R immunization leads to severe occlusive vasculopathy and most likely also PAH in the lung. Furthermore, evidence from a collaborative project with C. Hölscher suggests that the "first hit" may not necessarily be autoimmune in origin but may result from chronic infections of the lung and possibly also from chronic exposure to cigarette smoke. Our concept not only provides a new understanding of a modular structure of chronic lung diseases, but may represent an approach for new therapies in which deregulated cytokines are controlled in a symptom- and patient-dependent manner by appropriate biologicals.
Two-hit hypothesis for the disease manifestations in the lung
Identification of novel biomarkers and pathogenic drivers of tissue damage and remodeling in COPD
Neutrophil-epithelial interactions: Although the prominent role of neutrophils in the pathogenesis of COPD has long been recognized, the precise manner in which neutrophils carry out their function is not clear. We hypothesize that uncontrolled activation of neutrophils leads to chronic transition of airway epithelia to an inflammatory state, which conditions chronic inflammation, tissue damage, and peribronchiolar fibrosis. In this project, we are studying more than 50 isolated primary epithelial cell lines from clinically characterized patients with or without COPD at the functional and molecular levels.
Autoimmunity in COPD: Autoimmunity is a well-known but only partially understood process in the pathogenesis of COPD. Our previous results show that autoantibodies against extracellular antigens and neutrophil granular proteins are increased in patients with COPD compared to healthy controls. In this project, we are investigating autoantibodies against extracellular antigens to elucidate their role in COPD.