Priority Research Area Chronic Lung Diseases

Pulmonary Immune Diseases

Animal models for
Systemic sclerosis
Autoimmune bullous dermatoses
Allergic asthma
Lung function analyses
Ex vivo model for autoimmune bullous dermatoses
Protein biochemical methods
Cultivation of hybridomas, production and purification of monoclonal antibodies
Primary cell culture from human and animal tissues
Functional analyses of neutrophil granulocytes
In vitro differentiation and analysis of bone marrow-derived neutrophils, macrophages and dendritic cells
Isolation of organs from experimental mice, preparation of single cell suspensions
Magnetic cell sortingFlow cytometry and immunofluorescence
In-Cell Western Assay
Histological and immunohistochemical techniques
Quantitative RT-PCR, real time PCR
Detection of antibodies, cytokines and chemokines in tissue homogenates, supernatants and serum/plasma
Cytometric bead arrays
Determination of reactive oxygen metabolites (ROS)
Signal transduction