Priority Research Area Chronic Lung Diseases
Clinical and Molecular Allergology
Mission
Projects
Funding
Techniques
Conference Organization
Awards
Publications
Staff
Techniques
GENETIC METHODS
- Cloning and recombinant expression of allergens
- E. coli, "Clear coli"
- Pichia pastoris
- RNA-silencing of specific genes
- (real-time) PCR
ALLERGEN/PROTEIN PURIFICATION
- Aqueous and organic extraction methods
- Chromatography
- Affinity chromatography
- Ion exchange
- Hydrophobic interaction
- Reversed phase
- (Preparative) gel electrophoresis
- purification of lipophilic allergens
ALLERGEN/PROTEIN ANALYSIS
- Electrophoresis
- Isoelectric focusing (IEF)
- Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
- 2D-SDS-PAGE
- Difference gel electrophoresis (DIGE)
- Immunoblotting
- Detection of IgE, IgG und IgG subclasses
- Colorimetric readout
- Readout by chemiluminescence
- ELISA Techniques and ELISA establishment
- N-terminal sequencing
- Zymography
- Enzyme kinetics
- Epitope mapping of allergens
DESIGN OF PEPTIDE ARRAYS
MAPPING OF B CELL EPITOPES: IgE / IgG
ALLERGEN/PROTEIN ENGINEERING
- Protein fragmentation by protease digestion
- simulation of human digestion (Enzynorm/Kreon)
- Protein analysis: reduction / native analysis
- Controlled deglycosylation of proteins
- Enzymatic: Glycosidases
- Chemical: Periodate
CELL ANALYSIS / CELL CULTUE
- Cultivation of human / mammalian cells
- Cell analysis by flow cytometry (FCM, FACS)
- Analysis of cell activation by allergens (BAT, basophile activation test)
- Fluorescence microscopy, FRET techniques
FURTHER TECHNIQUES
- Generation of monoclonal antibodies and antisera (MAB technique)
- Immunohistochemistry (IHC)
- In silico protein-ligand docking studies (Autodock)