Priority Research Area Chronic Lung Diseases

Early Life Origins of CLD

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Techniques

Standard molecular and cellular methods are not detailed here (qPCR, Western blotting, flow cytometry, cell culture, histology  etc)

Special techniques

• Animal models
  • A: Exposure to cigarette smoke, mimicking active or passive smoking
  • B: Asthma models acute/chronic using HDM/ovalbumin
  • C: Transgenerational animal models: in utero smoking
    • A and B include online monitoring of total particulate matter and detailed phenotypic  and molecular characterization of mothers and offspring (cotinine levels, CO-Hb, invasive lung function testing, molecular networks and immunology, propensity to asthma development in offfspring )
• MicroRNA profiling in sera and tissues
• Air liquid interface culture of human bronchial cells with induction of asthma-like phenotype
• Isolation and functional characterization of Exosomes

For details of experimental protocols contact
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