Infection of primary cells with M. tuberculosis (Mtb) under BSL3 conditions in vitro
Magnetic isolation and characterization
Mtb-containing intracellular compartments
macropinosomes
Isolation und functional characterisation of lipid droplets
CRISPR/CAS9 in human immune cells
Transfection of human macrophages
Quantitative RT-PCR (Light Cycler)
ELISA
Histology and immunohistochemistry
Flow cytometry of mycobacteria and Mtb infected immune cells (BSL3)
Aerosol infection of immunocompetent and gene deficient mice with Mtb
Step wise identification of novel compounds with antimycobacterial activity
96-well format based anti-Tb activity tests using GFP- und mCherry-expr. Mtb (H37Rv)
Macrophage cytotoxicity tests using real time impedance measurements (xCelligence)
Activity tests using M. tuberculosis-infected human primary macrophages
Activity tests against drug susceptible, MDR and XDR-Mtb clinical isolates (in coop. with Dr. Doris Hillemann, National Reference Center) (MGIT format)
Projects