Priority Research Area Infections
Cellular Microbiology
Mission
Projects
Funding
Techniques
Publications
Staff
Techniques
Infection Biology
- Safety lab to work with BSL3 microorganisms such as Mycobacterium tuberculosis
- Aerosol infection model for tuberculosis (BSL3)
- Vaccine and anti-microbial drug testing
- Microbiome analysis (16s RNA sequencing, metagenomics, FISH, bioinformatics)
- In vivo and in vitro imaging (under BSL3 conditions)
- In vitro Infection models in primary cells from mouse and human (BSL3)
- In vivo infection models for Klebsiella pneumonia infection
- Stenotrophomonas maltophilia and mixed biofilm studies between bacteria and yeasts
- Air-liquid interface lung epithelial cell model for biofilm studies
- Bacteriophage collection
Infection Immunology
- FACS (BSL2 and 3)
- Histology Immunohistology
- Cytokine- and chemokine assays
- T-cell-Assays
- Quantitative Real-time PCR
- RNAseq
Cellular microbiology
- Intracellular trafficking studies of pathogens in host cells, confocal microscopy
- Live cell microscopy (BSL2 and 3)
- Primary cell cultures: human, mouse; macrophages, neutrophils, dendritic cells, lymphocytes, lung epithelial cells
- Subcellular fractionation, phagosome isolation
- Cell death assays
- siRNA-based knock-down
- Reporter strain of tuberculosis and S. maltophilia
Biochemistry
- SDS-PAGE, western blot
- Liposome technology
- Glycolipid isolation
- Thin layer chromatography