Priority Research Area Infections
Clinical Infectious Diseases
Mission
Projects
Funding
Techniques
Publications
Staff
Techniques
Bronchoscopic techniques for the diagnosis of pulmonary infections
- Bronchoalveolar lavages (BAL) for the preparation of cell suspensions (Endoscopy)
Cell biological methods
- Preparation of BAL for cytospins and BAL fluids (Immune Cell Analytics)
- Preparation of peripheral mononuclear cells (PBMNC)
- Elutriation for the preparation of monocytes (Immune Cell Analytics)
- In vitro infection of BAL cell suspensions with M. tuberculosis (Immune Cell Analytics, Microbial Inflammation Research)
- FACS-analysis for the detection of intracellular cytokines (Immune Cell Analytics)
- ELISPOT for the detection of IFN-g producing T-cells (Immune Cell Analytics)
- ELISA for the quantification of cytokines
- Multiplex-Analysis for the detection of max. 27 cytokines
- Quantitative RT-PCR for the detection of cytokine expression in BAL or PBMNC
- Immunohistochemistry for the intracellular detection of CXCL4 (PF4) (Pathology)
Translational studies
- Design, organization, coordination and analysis of international studies in collaboration with the TBNET