Priority Research Area Infections

Clinical Infectious Diseases

Bronchoscopic techniques for the diagnosis of pulmonary infections

Bronchoalveolar lavages (BAL) for the preparation of cell suspensions (Endoscopy)

Cell biological methods

Preparation of BAL for cytospins and BAL fluids (Immune Cell Analytics)
Preparation of peripheral mononuclear cells (PBMNC)
Elutriation for the preparation of monocytes (Immune Cell Analytics)
In vitro infection of BAL cell suspensions with M. tuberculosis (Immune Cell Analytics, Microbial Inflammation Research)
FACS-analysis for the detection of intracellular cytokines (Immune Cell Analytics)
ELISPOT for the detection of IFN-g producing T-cells (Immune Cell Analytics)
ELISA for the quantification of cytokines
Multiplex-Analysis for the detection of max. 27 cytokines
Quantitative RT-PCR for the detection of cytokine expression in BAL or PBMNC
Immunohistochemistry for the intracellular detection of CXCL4 (PF4) (Pathology)

Translational studies

Design, organization, coordination and analysis of international studies in collaboration with the TBNET