Priority Research Area Infections

Clinical Infectious Diseases

Mission    Projects    Funding    Techniques    Publications    Staff

Techniques

Bronchoscopic techniques for the diagnosis of pulmonary infections

  • Bronchoalveolar lavages (BAL) for the preparation of cell suspensions (Endoscopy)


Cell biological methods

  • Preparation of BAL for cytospins and BAL fluids (Immune Cell Analytics)
  • Preparation of peripheral mononuclear cells (PBMNC)
  • Elutriation for the preparation of monocytes (Immune Cell Analytics)
  • In vitro infection of BAL cell suspensions with M. tuberculosis (Immune Cell Analytics, Microbial Inflammation Research)
  • FACS-analysis for the detection of intracellular cytokines (Immune Cell Analytics)
  • ELISPOT for the detection of IFN-g producing T-cells (Immune Cell Analytics)
  • ELISA for the quantification of cytokines
  • Multiplex-Analysis for the detection of max. 27 cytokines
  • Quantitative RT-PCR for the detection of cytokine expression in BAL or PBMNC
  • Immunohistochemistry for the intracellular detection of CXCL4 (PF4) (Pathology)


Translational studies

  • Design, organization, coordination and analysis of international studies in collaboration with the TBNET