Priority Research Area Asthma and Allergy

Immunoproteasome

Mission    Project    Funding    Techniques    Publications    Staff

Techniques

  • Primary culture of human and mouse lung and immune cells
  • Biochemical analyses of proteasome activity (native gel, substrate-based activity tests and activity-based probe analysis)
  • Molecular biology-based methods (Western Blotting, immunoprecipitation, RTqPCR analysis, RNA seq, proteomics)
  • Cell biological assays for growth, survival, migration and differentiation
  • T-cell reporter assays
  • FACS analyses